Toll-like Receptor-4 Plays a Role in Macrophage Phagocytosis During Peritoneal Sepsis

LPS significantly increased the rate of phagocytosis by peritoneal macrophages from TLR4-WT mice, but not in those from TLR4-mutant mice, suggesting a role for TLR4 in phagocytosis. LPS also increased the rates of phagocytosis in cultured macrophages expressing TLR4, confirming these findings. The yield of gram-negative bacteria obtained from the peritoneal cavities of septic TLR4-WT mice was greater than that from TLR4 mutants, consistent with TLR4-dependent alterations in their septic course. Thus, in this paper. we conclude that TLR4 plays a critical role in the response to intraperitoneal E. coli through effects on phagocytosis by macrophages, suggesting the possibility of using TLR4 as a therapeutic target in diseases of peritoneal sepsis.
Above, the effect of LPS on the bacterial colonization of the intestinal barrier in TLR4-WT and TLR4-mutant mice. Toll-like receptor 4-WT and TLR4-mutant mice were injected with LPS or saline. Peritoneal lavage specimens were plated on gram-negative selective agar overnight, and bacterial growth was assessed. Representative of 5 separate experiments.

Anand RJ, Kohler JW, Cavallo JA, Li J, Dubowski T, Hackam DJ
J Pediatr Surg
2007 June
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January 19, 2011
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Last Update
January 19, 2011