Children's Hospital is part of the UPMC family.
Be safe anytime, anywhere.
To find a pediatrician or pediatric specialist, please call 412-692-7337 or search our directory.
A resource for our network of referring physicians.
For more information about research, please call our main office at 412-692-6438.
Children's Hospital is ranked One of America's Best Children's Hospitals.
We are studying memory Th17 responses using mouse models of Klebsiella pneumoniae.
Our previous work (Chen et. al., Immunity. 2011 Dec 23;35 (6):997-1009.) has demonstrated that immunization-induced Th17 cells provide serotype/antibody independent protection against a variety of strains of K. pneumonia including the recently described multidrug-resistant New Delhi metallo-beta-lactamase strain.
These Th17 cells recognized conserved Klebsiella outer membrane proteins (OMPs) and immunization with OMPs also conferred serotype/antibody independent protection.
Current work includes understanding of the mechanism of generation of these memory Th17 cells and cloning the highly conserved OMPs from K. pneumonia and testing their immunogenicity as Th17 based vaccine candidates.
Successful vaccine strategy can be used not only in prevention of multidrug resistant bacteria outbreaks but also in prophylactic treatment for immunocompromised patients that may lack B cell responses or for patients undergoing anti-CD20 treatment.
Vaccination generates memory Th17 cells in the lung (shown by flow cytometry) providing protection against bacteria including antibiotic-resistant strains.
Click to view larger image.
Cigarette smoke is known to cause several respiratory ailments, including chronic bronchitis, emphysema, chronic obstructive pulmonary disease (COPD) and lung cancer. We are investigating the role of cigarette smoke as a causative factor or modifier of these lung diseases.
Th17 cells are a critical component in chronic inflammatory diseases. Naive T cells differentiate into Th17 cells through co-exposure to IL-6 and TGF-β, both of which are present during COPD. Moreover, the aryl hydrocarbon receptor (AhR), which is activated by cigarette smoke, has a key role in promoting the Th17 response and expression of IL-22.
We are characterizing the role of IL-17R signaling in cigarette smoke-induced emphysema and IL-17 related cytokines as biomarkers in COPD.
Lung tissue samples from mouse models exposed to air and cigarette smoke showing IL-17RA-/- mice have significantly less tissue emphysema (D) compared to control mice (C). Click to view larger image.
Chronic alcohol consumption affects virtually all tissues of the body, leading to disorders in multiple organ systems. However, liver inflammation and fibrosis is a major cause of morbidity and mortality.
Although over expression of IL-22 can ameliorate hepatitis and improve hepatocyte survival, the protective mechanisms of IL-22 have not been fully elucidated. We hypothesize that IL-17 is critical for the development of alcoholic hepatitis and that endogenous IL-22 is rendered ineffective due to sequestration by Il-22RA2, an IL-22 binding protein.
Therefore, our current projects are: 1) Determine the cellular sources of IL-17A, IL-17F, and IL-22 in a murine model of alcohol-induced liver inflammation; 2) Investigate the contributions of hepatocyte expression of IL-17 receptors in mediating inflammation in our murine model of alcoholic hepatitis; and 3) Investigate the contributions of hepatocyte expression of IL-22 receptor and soluble IL-22RA2 in mediating inflammation and hepatocyte repair in a murine model of alcohol induced liver inflammation and in patients with alcoholic hepatitis.
Children's Hospital's main campus is located in the Lawrenceville neighborhood. Our main hospital address is:
Children’s Hospital of Pittsburgh of UPMC
One Children’s Hospital Way
4401 Penn Ave.
Pittsburgh, PA 15224
In addition to the main hospital, Children's has many convenient locations in other neighborhoods throughout the greater Pittsburgh region.
With myCHP, you can request appointments, review test results, and more.
For questions about a hospital bill call:
To pay your bill online, please visit UPMC's online bill payment system.
Interested in giving to Children's Hospital? Visit Children's Hospital of Pittsburgh Foundation's website to: