Core Facilities

Core Facilities laboratories, utilizing the latest analytical technologies, are currently focused on cell imaging, flow cytometry, histology and animal imaging. The laboratories are available to the many pediatric basic research teams based at the John G. Rangos Sr. Research Center and are also available to other researchers from beyond the hospital system. Information about the labs, including contacts and links to PDFs of services descriptions and usage guidelines, are provided below.

Cell Imaging

Equipped with confocal microscopes and devices for live cell imaging and laser capture microdissection.

Cell Imaging Core I Laboratory
5th Floor, Rangos Research Center
Director: George Gittes, MD
Contact: Prasadan, Krishna
Krishna.Prasadan@chp.edu
412-692-9223

Cell Imaging I Facility Services and Usage Guidelines (PDF)

Cell Imaging Core II Laboratory
7th Floor, Rangos Research Center
Director: Tunda Hidvegi, PhD
Contact: Houming Cai, caih@chp.edu  
412-692-3547

Cell Imaging II Facility Overview of Services (PDF)

Flow Cytometry

Analytical flow cytometry and sterile cell sorting equipment and services.

Flow Cytometry Core Laboratory
8th Floor and 6th Floor, Rangos Research Center
Director: Abbe deVallejo, PhD
Contacts: Joshua Michel, joshua.michel@chp.edu and Alison Logar, ajs98@pitt.edu
8th Floor:412-692-6968
6th Floor: 412-692-3025

Flow Cytometry I Facility Services and Usage Guidelines (PDF)

Genetic Sequencing

Offering Illumina next-generation sequencing services, including DNA and RNA extraction, sequencing and up-front services.

Health Sciences Sequencing Core
8th Floor, Rangos Research Center, Room 8145
Director: Amanda Poholek, PhD
Contact: Will MacDonald  
w.a.macdonald@pitt.edu
412-692-3555

Get more information about this core facility.
Guide to Next Generation Sequencing 

Histology

Support to investigators in need of histology-based technical services and pathological interpretation.

Histology Core Laboratory
3537 Rangos Research Center 
Director: Sunder Sims-Lucas, PhD, sunder.sims-lucas@chp.edu
Contact: Michele Mulkeen, mlm159@pitt.edu
412-692-6856

Histology Overview of Services (PDF)

Metabolic Core

Analytical services to support research in energy metabolism, metabolic pathways, and cellular metabolic function including mass spectrometry (LC/MS/MS), Seahorse® analyzer, amino acid analyzer, and reverse-phase HPLC system.

Metabolic Core Laboratory
5th Floor, Rangos Research Center, Room 5156
Director: Clinton Van’t Land, PhD
Contact: Clint Van’t Land, clinton.vantland@chp.edu
412-692-7652

Animal Imaging

Including “micro” magnetic resonance imaging (MRI) and Computer Tomography (CT) systems, high-resolution ultrasound and bioluminescence systems.

Animal Imaging Core Laboratory
4th Floor, Rangos Research Center
Director: Yijen Wu
Contact: Yijen Wu, yijen.wu@chp.edu 
412-692-9902

Animal Imaging Facility Services and Usage Guidelines (PDF)